Dana Rohanová, PhD

Associate professor, Department of Glass and Ceramics, University of Chemistry and Technology Prague

Characterisation of the surface reactivity of bioactive glass nanoparticles

Collaborators: Andrada Damian-Buda, Prof. Aldo R. Boccaccini

In this project we will collaborate on the in vitro testing of bioactive materials with focus on highly reactive bioactive glass nanoparticles. One of the most important task will be setting up the in vitro test conditions of such a sophisticated form of material. Different testing solutions, including Simulated body fluid (SBF), will be used. Sol-gel derived bioactive glass nanoparticles will be characterized with XRD, SEM/EDS and XRF before, during and after the in vitro test. The SBF eluates will be analysed using ICP-OES. This work continues our efforts to set up the most suitable conditions for testing various forms and types of glassy biomaterials and to improve the existing ISO standard, as documented in previous publications (for example [1], [2]).

[1] D. Rohanová et al., Is non-buffered DMEM solution a suitable medium for in vitro bioactivity tests?  J. Mater. Chem. B, 2 (2014) 5068-5076.  DOI:  https://doi.org/10.1039/C4TB00187G

[2] D. Rohanová et al., Interaction of HEPES buffer with glass-ceramic scaffold: Can HEPES replace TRIS in SBF?,  Journal of Biomedical Materials Research – Part B 106 (2018) 143-152. DOI: 10.1002/jbm.b.33818