Dian Wang

Former Master student

3D biofabrication of hydrogels based on oxidized hyaluronic acid

 

Supervisors: Sonja Kuth, Prof. Aldo R. Boccaccini

Hyaluronic acid (HA) is a linear polysaccharide present in the human extracellular matrix (ECM). HA has biocompatibility and water holding capacity, therefore HA-based hydrogels have been widely used in tissue engineering [1]. However, the applications of HA hydrogels are limited by their poor mechanical properties and higher rate of hydrolytic degradation. Oxidation of HA with Sodium metaperiodate (NaIO4) can form oxidized HA (OHA). The multiple aldehyde groups contained in OHA can easily react with materials containing amino functional groups to form Schiff base linkage, thus further forming OHA-based hydrogel scaffolds [2, 3].
Extrusion 3D printing it is a manufacturing technology for constructing biological scaffolds with complex functions under computer-aided design [3]. In the research we aim to use 3D printing to prepare OHA/gelatin-based hydrogel scaffolds for tissue engineering.

[1] X. Xu, A.K. Jha, D.A. Harrington, M.C. Farach-Carson, X. Jia, Hyaluronic acid-based hydrogels: from a natural polysaccharide to complex networks, Soft Matter 8 (2012) 3280–3294

[2] Ashiq Hussain Pandit a, Nasreen Mazumdar b, Sharif Ahmad. Periodate oxidized hyaluronic acid-based hydrogel scaffolds for tissue engineering applications, Biological Macromolecules 137 (2019) 853–869

[3] S. Kuth, et al., Oxidized Hyaluronic Acid-Gelatin-Based Hydrogels for Tissue Engineering and Soft Tissue Mimicking, Tissue Eng. Part C: Methods 28 (2022) 301-313.

[4] Wang Xue-xin, Zhang Ming-jian, Li Xiao-bing, Han Wei-xin, Li Jin-qiao.Three-dimensional bioprinting of tissue/organ analogues: a review on techniques, materials and processes. Chinese Journal of Tissue Engineering Research, 2018, 22(10): 1611-1617.