Jan Novak

Masterstudent

Microsphere-based pre-crosslinking of ADA-GEL bioinks for improved printability and cell viability in biofabrication applications

 

Supervisors: Jonas Roeder, Prof. Aldo R.Boccaccini

Alginate dialdehyde-gelatin (ADA-GEL) hydrogel has emerged as a promising material in the field of biofabrication owing to its versatile properties and biocompatibility [1]. However, the existing challenges of low shape fidelity post-printing and the need for subsequent crosslinking restrict its full potential. One suggested solution to overcome these limitations is the pre-crosslinking of the bioink prior to printing. A previous study has explored the use of calcium carbonate particles to enhance printability, but this approach introduced new challenges, including pH shift and reduced cell viability [2]. This master’s thesis aims to develop a novel approach for pre-crosslinking ADA-GEL by incorporating calcium-crosslinked ADA microspheres into the bioink, thereby potentially improving the performance of ADA-GEL hydrogel for advanced biofabrication applications.

[1] Aylin Kara Özenler et al 2023 Biofabrication 15 025012 10.1088/1758-5090/acb6b7

[2] Jonas Hazur et al 2020 Biofabrication 12 045004 10.1088/1758-5090/ab98e5